ABCB4 Knockout cell line (IPSC)

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ABCB4 Knockout cell line (IPSC) Order

Catalog#

YKO-H1186

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

ABCB4 Knockout cell line (IPSC)

Species

Human

Gene Name

ABCB4

Gene ID

5244

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Target region

Knockout region: Exon 4 (based on transcript ABCB4-212)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ABCB4 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the IPSC cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ABCB4 gene knockout ABCB4 Knockout cell line (IPSC) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

ABCB4 Knockout cell line (IPSC)

Catalog#

YKO-H1186

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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ABCB4 Knockout cell line (IPSC)

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